Application

Suggested dilution for most applications: 1:50-1:200 for most applications.

Optimal working dilutions must be determined by the end user.

Detect Rabbit IgG using this Goat anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed validated for use in IF.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05 Sodium Azide.

RECONSTITUTION:

Reconstitute to 1 mg/mL with sterile distilled water.

Specificity

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

FLUOROPHORE/ PROTEIN ABSORBANCE RATIO

Tetramethyl Rhodamine Isothiocyanate A550/A280 = 0.54